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Electron microscopy EM is a technique for obtaining high resolution images of biological and non-biological specimens. It is used in biomedical research to investigate the detailed structure of tissues, cells, organelles and macromolecular complexes. The high resolution of EM images results from the use of electrons which have very short wavelengths as the source of illuminating radiation. Electron microscopy is used in conjunction with a variety of ancillary techniques e.
During the last 70 years, transmission electron microscopy TEM has developed our knowledge about ultrastructure of the cells and tissues. Another aim is the determination of molecular structure, interactions and processes including structure-function relationships at cellular level using a variety of TEM techniques with resolution in atomic to nanometre range. Even with the best transmission electron microscope, it is impossible to obtain real results without optimal sample preparation, respecting both the structure and the antigenicity preservation. Preparation techniques for high-resolution study of both macromolecular complex and organelles within cellular complex are based on fast cryoimmobilisation process, where the sample is in the most native, hydrated state. Next, thin samples are directly visualised under cryo-transmission electron microscopy cryo-TEM , while thicker samples require a thinning step via cryo-electron microscopy of vitreous sections CEMOVIS or cryo-focused ion beam cryo-FIB before visualisation. Alternatively, vitrified samples are freeze substituted and embedded in chosen resin for room temperature ultramicrotomy. This preparation technique is suitable for morphological study, 3D analysis of cellular interior and immunoelectron microscopy.
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Nigel Chaffey, Hayat MA. Principles and techniques of electron microscopy: biological applications. Cambridge: Cambridge University Press. Oxford University Press is a department of the University of Oxford. It furthers the University's objective of excellence in research, scholarship, and education by publishing worldwide. Sign In or Create an Account. Sign In.
Principles and techniques of electron microscopy vol 2 biological applications. Hayat, M. Biological Applications. Xv p. Xv, Principles and techniques of electron microscopy.
Principles and Techniques of Electron Microscopy: Biological Applications Get a printable copy (PDF file) of the complete article (K), or click on a page.
Synopsis: Principles and Techniques of Electron Microscopy is the standard work for biological electron microscopists wishing to learn how to prepare their specimens for electron microscopic investigation. This fully revised and expanded fourth edition includes three new chapters covering such topics as plant tissues, immunocytochemistry, and applications of microwave irradiation to microscopy. It provides practical instructions on how to process biological specimens, as well as a detailed discussion on the principles underlying the various processes. Hayat presents methods in a self-explanatory form and includes alternative procedures and points of disagreement to help the reader interpret data accurately.
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The advantage of LM is that it can provide wide field images of whole, often living, cells, but its resolution is limited.Reply
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Hayat MA. Principles and techniques of electron microscopy: biological applications. 4th edn. PDF; Split View. Views. Article contents.Reply